منابع مشابه
Chicken globin gene number.
Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DN...
متن کاملChicken /3-Globin Genes
We reported previously that DNA bend sites appear in the human /3-globin locus at an average distance of 680 bp. The relative locations of the sites were conserved among the five active /3-like globin genes and one pseudogene. Here, we mapped the sites in the /3-like globin genes from various species and examined their conservation. The locations of the bend sites in the bovine, rabbit and chic...
متن کاملAlpha globin gene number: population and restriction endonuclease studies.
Restriction endonuclease analysis was used to test a proposed genetic model using alpha-globin gene number to account for the observed distributions of the proportions of hemoglobin (Hb) S in sickle cell trait. In a subsample of specimens collected during a population survey in India, these studies confirmed that the postulated genotype was present in 22 of the 23 individuals examined. In the s...
متن کاملRelease of a globin gene enriched chromatin fraction from chicken erythrocyte nuclei following DNase II digestion.
Mild digestion of chicken erythrocyte nuclei with deoxyribonuclease II results in the release of a chromatin fraction which is 4- to 13-fold enriched for the globin coding sequences when compared to total chicken DNA. The remaining nuclear pellet is depleted in these sequences. A maximum of 25% of the globin genes have been recovered in the released fraction. The addition of 5 mM sodium butyrat...
متن کاملRepeated sequence organization and RNA transcription map of the chicken adult beta-globin gene region.
We have used adaptations of the contact hybridization method to determine the repeated sequence map of a cloned fragment of chicken genomic DNA and to establish the map of RNA transcription in vivo from this same fragment. The cloned 6.2-kilobase pair (kbp) Eco RI fragment contains the adult beta-globin gene and a portion of the embryonic epsilon-globin gene. The technique of contact hybridizat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1976
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/3.8.2019